Features:

• Universal– can be used for both cell and tissue samples from a wide range of sources: mammalian blood, mammalian cell cultures, mammalian tissues, insect, yeast, bacteria.
• Efficient– high yields of total RNA.
• Fast– 15 minutes after lysis step.
• Pure– an A_260/280 ratio > 1.9.
• Convenient– spin columns are capped and assembled with collection tubes.

Applications:

1. Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:
   – RT-PCR,
   – RT-qPCR,
   – Northern blotting,
   – nuclease protection assay.

Description:

The GeneJET™ RNA Purification Kit is a simple and efficient system for purification of total RNA from mammalian cultured cells, tissue, human blood cells, bacteria and yeast.

The kit utilizes a silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious cesium chloride gradients, alcohol precipitation or toxic phenol-chloroform extractions.

RNA molecules longer than 200 nucleotides can be isolated with the GeneJET™ RNA Purification Kit in 15 minutes after the lysis step. The high-quality purified RNA can be used in a wide range of downstream applications including RT-PCR, RT-qPCR, Northern blotting and other RNA-based analyses.

Quality Control:

The GeneJET™ RNA Purification Kit is qualified by isolating total RNA from 20 mg of mouse muscle or heart following the described protocol. The purified RNA has an A_260/280 ratio of ≥1.9. The RNA integrity numbers (RIN) of RNA purified from mouse muscle and heart are >7 and >8, respectively. Functional quality of the purified RNA is evaluated by RT-PCR of mouse dystrophin RNA resulting in a 13.3 kb PCR product.

Components:

• Proteinase K
• Lysis Buffer
• Wash Buffer 1 (concentrated)
• Wash Buffer 2 (concentrated)
• Water, nuclease-free
• GeneJET™ RNA Purification Columns (pre-assembled with Collection Tubes)
• Collection Tubes (2 ml)
• Collection Tubes (1.5 ml)
• Detailed Protocol