BioPhotometer Plus

24-01-2011

 

With the BioPhotometer plus, Eppendorf offers a compact UV/Vis photometer for use in molecular biology, biochemistry and cell biology.

 

 

The photometer provides instant, out-of-the-box access to 32 routine methods, of which 9 methods are freely programmable, e. g.:

  • Measurement of DNA, RNA and protein concentration (UV and colorimetric)
  • Incorporation rate of fluorescent molecules (550 nm/650 nm), e. g., for microarray experiments
  • Enzymatic assays (e.g., peroxidase, alkaline phosphatase, β-galactosidase)
  • Optical density of cells (OD 600)

 

Both measurement and calculation of results are performed at the press of a button, and the results of the analysis, as well as all accompanying data, are available at a glance. This guarantees safe, error-free operation and reliable results.

 

In addition to its low weight, the photometer is small, yet extremely sturdy due to a robust metal housing, which allows for easy transport and cleaning.

 

The BioPhotometer plus is designed for use with cuvettes such as the Eppendorf UVette®, but is also optimized for measurement of small volumes through the use of microliter cells such as Hellma® TrayCell.

 

The new BioPhotometer data transfer software can now be used to transfer all measurement and calibration data to a PC. Besides enabling the printing of results it also allows results to be exported to data processing software for further evaluation. The program also offers the option of data archiving, which can be clearly configured with an optional user administration.

 

Applications

  • Quantification of nucleic acids (dsDNA, ssDNA, RNA, Oligo)
  • Quantification of proteins (280 nm, Bradford, Lowry, BCA)
  • Cell density measurement (OD600)
  • NEW - Incorporation rate of fluorescent dyes (dye 550/650, e.g., Cy-3, Cy-5)
  • NEW - Enzymatic assays: endpoint detection (e.g., of enzyme activities or cell biological assays)
  • NEW - Direct absorbance measurement of single wavelengths without any calculation (230 nm, 260 nm, 280 nm, 340 nm, 405 nm, 490 nm, 550 nm, 595 nm, 650 nm)

 

Features

  • 9 wavelengths suitable for the common methods in molecular biological, biochemical and cell biological labs
  • Preprogrammed methods for quantification of nucleic acids and proteins as well as incorporation rate of fluorescent dyes
  • Automatic calculation of dilution factors
  • Assays at 340 nm, 405 nm, and 490 nm are user defined for highest flexibility
  • Measurement of single wavelengths without any calculation
  • Storage of the last 100 results and all corresponding data
  • Simple user guidance for error-free operation
  • Different colored function keys for easy operation
  • Compact design and robust housing
  • Stand-alone unit without need for PC connection
  • No pre-warming saves time
  • Xenon flash lamp with long lifetime and high light intensity
  • No moving parts – less service needs